Food safety, which is regarded as a global and expensive health problem, is often threatened with the entry of microorganisms, which reduce their storage time and cause acute illness. Another important global problem is the corrosion of metal and plastic structures, which is caused by various colonies of bacteria. The use of microorganisms that cause the biodegradation of materials is promising for solving the problem of environmental pollution with polymeric materials. That is, the problem of developing methods and markers for identifying microorganisms with high sensitivity and reproducibility is relevant. Thus, the actual task is a developing of methods of detection with high sensitivity, reproducibility.
The article presents the results of recognition and labeling of bacteria with a block-copolymer with fragments of oligonucleotide and fluoroalkyl alcohol, using luminescence spectroscopy and mass spectroscopy of secondary ions. Obtaining a hybrid block-copolymer with an oligonucleotide block was carried out in two stages. A fluorine-containing surface-active polymer with a terminal functional epoxy group was obtained at the first stage. At the second stage, an oligonucleotide which contains the primary amino group was bound with epoxy group of polymeric block. The first block was obtained by radical polymerization of N-vinylpyrrolidone (NVP). The process was initiated by the redox system of cerium salt - fluoroalkyl alcohol. An epoxy-containing cumine derivative was used as a chain transfer agent for control the colloid-chemical characteristics of the products and the introduction into their composition of the terminal functional epoxy fragment.
The effect of the length of hydrophobic fluoroalkyl and hydrophilic oligo(NVP) fragments on the size of micelles formed by oligomers in water was studied by dynamic light scattering. It was established that the size of oligomer particles below the CMC point increases with increasing a length of the fluoroalkyl fragment, which is not observed for concentrations above the CMC point. The observed effect is explained by the different ability of the fluoroalkyl fragment to be compacted inside the micellar core for different concentrations of oligomers in the solution.
The detection of bacteria in luminescent light and by secondary ion mass spectroscopy confirmed the possibility of using fluoride-based hybrid block-copolymer (NVP) with oligonucleotide block as a bacterial label.
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