High Performance Liquid Chromatographic Determination of Bupropion Using H-Clinoptilolite as a Sorbent for Plasma and Urine Purification

Authors: 
Iryna Halkevych, Liudas Ivanauskas, Yuriy Bidnychenko and Zita Barsteigiene

Iryna Halkevych-1, Liudas Ivanauskas-2, Yuriy Bidnychenko-1 and Zita Barsteigiene-2

  1. Danylo Halytsky Lviv National Medical University, 69, Pekarska str., 79010 Lviv, Ukraine; iryna.galkevych@gmail.com
  2. Lithuanian University of Health Sciences, Medical Academy, Faculty of Pharmacy, 9, A.Mickeviciaus str., LT 44307, Kaunas, Lithuania

The coupling of solid-phase extraction (SPE) using H-clinoptilolite as an adsorbent with a high performance liquid chromatography (HPLC) method was developed for the high efficiency enrichment and rapid determination of bupropion in plasma and urine. The effect of the adsorbent particles size and pH on adsorption behavior of bupropion to H-clinoptilolite was evaluated. The experimental conditions of the sorbent preparation were developed, the effective solvents for the sorbent conditioning were chosen. The optimal eluent for bupropion extraction by H-clinoptilolite was chosen.

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