method validation

Preparation of Secondary pH of Phthalate Buffer Solution Using Differential Potentiometric Cell: Method Validation and Application

A secondary pH of phthalate buffer was prepared by differential potentiometry method using a Baucke cell which is separated by sintered glass disk in the middle of cell – so called two-half cells. The method has been validated at 298 K with the purpose to evaluate its suitability for meeting the application requirement. The method validation parameters include accuracy (bias), precision (repeatability), and estimation of measurement uncertainty. It was found that both accuracy and precision of the method were good, which is indicated by their very low standard deviation (SD).