CHARACTERIZATION OF THE “ENZYMNYI” DETERGENT ACCORDING TO ABILITY OF MICROBIAL BIOFILMS DISRUPTION

2018;
158-164
1
Ternopil Ivan Puluj National Technical University
2
Ternopil Ivan Puluj National Technical University
3
Ternopil Ivan Puluj National Technical University
4
Lviv Polytechnic National University
5
Lviv Polytechnic National University

The article contains the results of the research on the influences of different concentrations of working solutions of the enzyme product «Enzymnyi» on microbial biofilms Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus cereus. Research has shown that the optimal effect of the detergent on the biofilm is visible with a concentration of 0.01%, where the biofilms optical density is decreased by a factor of 3.7 in Staphylococcus aureus and in Pseudomonas aeruginosa and times  4.5 in Bacillus cereus in comparison with the control and is within 0,7-0,53 units, respectively. It has been found that the optical density of microbial biofilms is decreasing with decreasing of concentration. With an increasing of the temperature of the «Enzymnyi» detergent to 60° С with the exposition of 30 minutes density of Bacillus cereus was 0.39 units, which is in 4.5 times less in comparison with control, density of Pseudomonas aeruginosa biofilms was reduced in 4.4 times and reached 0.4 units, а density of Bacillus cereus biofilms – in 4,5 times and reached 0,39 units. The authors point the time-dependent effect of the «Enzymnyi» detergent, in other words, with increasing of the exposition, there is a greater degradation of biofilms formed by Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus cereus.

At the same time, with the increasing of the temperature of the «Enzymnyi» detergent to 60 °С and the exposition of 60 minutes  there is the increasing of the its effectiveness in terms of the bacteria disruption in biofilms.

1. Дегтерев Г. П. Качество молока в зависимости от санитарного состояния доильного оборудования / Г. П. Дегтерев // Молочная промышленность. − 2000. − № 5. − С. 23−26. 2. Кухтин М. Д. Формування мікробних біоплівок на поверхнях різних матеріалів мікроорганізмами, 164 які виділені з технологічного устаткування / М. Д. Кухтин, Ю. Б. Перкій, Н. В. Крушельнецька // Ветеринарна біотехнологія. − Ніжин: ПП Лисенко М. М., 2013. − № 22. − С. 292−297. 3. Yung-Hua Li. Xiaolin Tian. Quorum Sensing and Bacterial Social Interactions in Biofilms // Sensors. – 2012. – N 12. – P. 19–38. 4. Davey M. E. Microbial biofilms: from ecology to molecular genetics [Теxt] / M. E. Davey, G. O. O’Tool // Microbiol. Mol. Biol. Rev. – 2000. – 64, N 9. – P. 847–867. 5. Formation of biofilms on dairy equipment and the influence of disinfectants on them / M. Kukhtyn, O. Berhilevych, K. Kravcheniuk, O. Shynkaruk, Y. Horiuk, N. Semaniuk // Eastern-European Journal of Enterprise Technologies. – 2017. – Vol. 5/11. – P. 26–33. 6. The influence of disinfectants on microbial biofilms of dairy equipment / M. Kukhtyn, O. Berhilevych, K. Kravcheniuk, O. Shynkaruk, Y. Horiuk, N. Semaniuk // “EUREKA: Life Sciences”. – 2017. – Vol. 5. – P. 11–17. 7. Involvement of a pasteurizer in the contamination of milk by Bacillus cereus in a commercial dairy plant / B. Svensson, A. Eneroth, J. Brendehaug, G. Molin, A. Christiansson // J. Dairy Res. – 2000. – Vol. 67. – P. 455–460. 8. Quantitative analysis of survival of Staphylococcus aureus or Listeria innocua on two types of surfaces: Polypropylene and stainless steel in contact with three different dairy products / N. Oulahal, W. Brice, A. Martial, P. Degraeve // Food Cont. – 2008. – Vol. 19. – P. 78–185. 9. Alginate overproduction affects Pseudomonas aeruginosa biofilm structure and function / M. Hentzer, G. M. Teitzel, G. J. Balzer, A. Heydorn, S. Molin, M. Givskov and M. R. Parsek // J. Bacteriol. – 2001. – Vol. 183 (18). – P. 5395– 5401. 10. Using enzymes to remove biofilms of bacterial isolates sampled in the food-industry / Yannick Lequette, Gauthier Boels, Martine Clarisse, Christine Faille // Biofouling. – 2010. – Vol. 26, N 4. – Р. 421−431.