This work investigated the possibility of introducing Echinacea purpurea into in vitro culture in order to obtain callus biomass. The method of seed sterilization and seedling cultivation on Murashige-Skoog medium was described. The effect of various combinations of growth regulators (IAA, BAP, NAA, 2,4-D) on callus formation from leaf, stem and root explants was experimentally determined. It was found that the formation of callus biomass occurs better on a medium with the addition of growth regulators IAA and BAP in a ratio of 2:1 mg/l than NAA, 2,4-D in a ratio of 1:1 mg/l. Callus of yellow-green and light brown color was formed on leaf and stem explants. Root explants did not show callusogenesis.
- Mamedova, V. N. (2023). Advantages of Growing Plants by in-Vitro Method. International Journal of Scientific Trends, 2(4), 19–23. Retrieved from https://scientifictrends.org/index.php/ijst/article/view/86
- Kulak, V.; Longboat, S.; Brunet, N.D.; Shukla, M.; Saxena, P. (2022) In Vitro Technology in Plant Conservation: Relevance to Biocultural Diversity. Plants, 11, 503. https://doi.org/10.3390/ plants11040503
- Anjali, Kopal Singh, Dr. Gurshaminder Singh (2024). Fundamental of Tissue Culture and it’s Future Prospects in Crop Improvement. International Journal of Scientific Research & Engineering Trends, 10 (5), ISSN (Online): 2395-566X
- Burlou-Nagy C., Banica F., Jurca T., Vicas L.G., & Marian E., et al. (2022) Echinacea purpurea (L.) Moench: Biological and Pharmacological Properties - A Review. Plants., 11, 1244. doi:10.3390/plants11091244
- Ciganovic P., Jakupovic L., Momchev P., Nižic Nodilo L., Hafner A. & Zovko Koncic M. (2023) Extraction Optimization, Antioxidant, Cosmeceutical and Wound Healing Potential of Echinacea purpurea. Glycerolic Extracts. Molecules, 28, 1177. doi:10.3390/molecules28031177
- El-Ashmawy, N., El-Zamarany, E., Salem, M., El-Bahrawy, H., & Al-Ashmawy, G. (2015). In vitro and in vivo studies of the immunomodulatory effect of Echinacea purpurea on dendritic cells. Journal of Genetic Engineering and Biotechnology, 13(2), 185–192. doi: 10.1016/j.jgeb.2015.05.002
- Li, T. S. C. (1998). Echinacea: Cultivation and Medicinal Value. HortTechnology, 8(2), 122–129. https://doi.org/10.21273/horttech.8.2.122
- Nilanthi, D., Chen, X.-L., Zhao, F.-C., Yang, Y.-S., & Wu, H. (2009). Induction of Tetraploids from Petiole Explants through Colchicine Treatments inEchinacea purpureaL. Journal of Biomedicine and Biotechnology, 2009, 1–7. https://doi.org/10.1155/2009/343485
- Pospelov, S., Zdor, V., Mishchenko, O., Pospelova, A., & Kovalenko, N. (2020). Model of creation of productive agrocenosis of Echinacea. E3S Web of Conferences, 222, 02048.
- Makukha, O. (2021). Technological Improvement of Echinacea purpurea Cultivation. Ecological Engineering & Environmental Technology, 22(5), 89–96. https://doi.org/10.12912/27197050/139336
- Coker P.S., & Camper N.D. (2000) In vitro culture of Echinacea purpurea L. J. of Herbs Spices an Medicinal Plants, 7(4), 1-7. doi:10.1300/J044v07n04_01
- Kristen L. Choffe, Jerrin M. R. Victor, Susan J. Murch & Praveen K. Saxena. (2000) In Vitro Regeneration of Echinacea purpurea L.: Direct Somatic Embryogenesis and Indirect Shoot Organogenesis in Petiole Culture / In vitro Cellular & Developmental Biology. Plant, 36, 1, 30-36. doi:10.1007/s11627-000-0008-4
- Dahanayake, N., Chen, X.-L., Zhao, F.-C., & Yang, Y.-S. (2011). An Efficient In Vitro Propagation Culture Protocol for Purple Coneflower (Echinacea purpurea L.). Tropical Agricultural Research and Extension, 13(2)29–32. doi: 10.4038/tare.v13i2.3135
- Maggini, V., Mengoni, A., Gallo, E. R. G., Biffi, S., Fani, R., Firenzuoli, F., & Bogani, P. (2019). Tissue specificity and differential effects on in vitro plant growth of single bacterial endophytes isolated from the roots, leaves and rhizospheric soil of Echinacea purpurea. BMC Plant Biology, 284(2019), 30-36. doi: 10.1186/s12870-019-1890-z