1. JCCT
  2. All Volumes and Issues
  3. Volume 17, Number 3, 2023
  4. Physico-Chemical Studies of the Interaction Mechanism of Double and Trivalent Iron Double Oxide Nano-Particles with Serpin Protein Ovalbumin and Water

Physico-Chemical Studies of the Interaction Mechanism of Double and Trivalent Iron Double Oxide Nano-Particles with Serpin Protein Ovalbumin and Water

JCCT.
2023;
Volume 17, Number 3
: pp.481 - 494
https://doi.org/10.23939/chcht17.03.481
Authors:
  1. Iryna Tsykhanovska
  2. Mykola Riabchykov
  3. Alexandr Alexandrov
  4. Victoria Evlash
  5. Oksana Bryzytska
  6. Sergey Gubsky
  7. Tatyana Lazareva
  8. Olga Blahyi
1
Ukrainian Engineering Pedagogic Academy
2
Lutsk National Technical University
3
Ukrainian Engineering Pedagogic Academy
4
Kharkiv State University of Food Technology and Trade
5
National University of Pharmacy
6
State Biotechnological University
7
Ukrainian Engineer Pedagogic Academy
8
Ukrainian Engineer Pedagogic Academy

The novelty of the work is the theoretical justification and experimental confirmation of the mechanism of interaction of Fe3O4 nanoparticles with Н2О and ovalbumin-OVA, which was carried out with the help of a complex of physical and chemical studies. It was determined that the mechanism is based on the clustero-philicity of nanoparticles and hydrogen, electrostatic and van der Waals interactions. It was established that the interaction of Fe3O4 nanoparticles with OVA took place by the mechanism of static quenching with the formation of an intermolecular non-fluorescent complex that changes the native structure of OVA. The binding constant varied from 3.3×105 to 4.8×105 L•mol-1 depending on the pH value of the medium and temperature. Thermodynamic calculations confirmed the spontaneity of the binding process with the predominance of the enthalpy factor.

Fe3О4 nanoparticles
water
OVA
binding
water absorption
water retention
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