Hydrotrope-Induced Micellar Phase of Sodium Dodecyl Slfate as New Detergent for Extraction and Stabilization of Proteins
X-ray phase analysis and IR spectroscopy data have confirmed a gel-like nature of sodium dodecyl sulfate micellar phase induced by sodium chloride and salicylic acid additives. This phase essentially consists of water (ωH2O > 50 %), which is connected with main components of the micellar-extraction system through intermolecular hydrogen bonds. Capacity of such micellar phase to quantitatively extract of proteins (R > 94 %) with saving of their catalytic activities and dispersity was found.