In the paper, a comparative photochemical analysis of ethanol extracts of Echinacea purpurea (with a concentration of 40, 70, and 90%) was carried out. The total content of polyphenolic compounds (0.001-0.713 mg-eq) and flavonoids (0.667-2.873 mg-eq in terms of rutin and quercitin) was determined by spectrophotometry, and the maximum yield of active substances was recorded for 70% ethanol.
This work investigated the possibility of introducing Echinacea purpurea into in vitro culture in order to obtain callus biomass. The method of seed sterilization and seedling cultivation on Murashige-Skoog medium was described. The effect of various combinations of growth regulators (IAA, BAP, NAA, 2,4-D) on callus formation from leaf, stem and root explants was experimentally determined. It was found that the formation of callus biomass occurs better on a medium with the addition of growth regulators IAA and BAP in a ratio of 2:1 mg/l than NAA, 2,4-D in a ratio of 1:1 mg/l.