ANTIOXIDANT EFFECT OF HUMIC ACIDS ON RAT HEPATOCYTES

2021;
: 64-71
1
Lviv Polytechnic National University
2
Lviv Polytechnic National University
3
Department of PhChFF InPOCC NAS of Ukraine
4
Institute of Physical-Organic Chemistry and Coal Chemistry named after L. M. Lytvynenko of the National Academy of Sciences of Ukraine
5
Lviv Polytechnic National University
6
Lviv Polytechnic National University

The antioxidant properties of humic acids of various origins have been investigated (from bio-humus, black soil from Ternopil region, and peat from different regions, such as: Velykyi Liubin, Drohobych and Belarus Republic). Titrimetric analysis has been shown the quantitative composition of the acidic functional groups of the obtained humic acids. According to the data of thermogravimetric analysis, the values of parameters, showing the ratio of their aliphatic to the cyclic part were determined. It was found that all obtained humic acids are characterized by high antioxidant activity: the formation of thiobarbituractive products and carbonyl groups of proteins in liver hepatocytes were decreased. The inhibition of the products of free radical formation of lipid components and proteins was better inhibited by 1% solutions of humates from vermicompost and humic acids from peat. The results indicate prospects of the humic acids for creating the means of restoring the physiological functions of biological objects in pathological conditions under conditions of free radical stress for application in cosmetic pharmaceutical products.

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