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  4. Еffect of downstream processing on content of glutathione and cell viability during production of inactive dry yeast

Еffect of downstream processing on content of glutathione and cell viability during production of inactive dry yeast

CTAS.
2025;
Volume 8, Number 1
Authors:
  1. M. H. Stets
  2. V. A. Yerokhin
  3. V. V. Havryliak
  4. Vira Lubenets
  5. B. Pilarczyk
1
Lviv Polytechnic National University
2
Lviv Polytechnic National University
3
Lviv Polytechnic National University
4
Lviv Polytechnic National University
5
West Pomeranian University of Technology in Szczecin, Poland

Glutathione (GSH) is a tripeptide thiol with important protective activity in living organisms. Due to its ability to prevent oxidation, this substance is commonly used in biotechnological products. The fed-batch process with yeasts of the genus Saccharomyces coupled with downstream processing to obtain inactive dry yeasts with high GSH content is the common method for industrial production. Short-term high-temperature treatment (1 minute at 105°C) emerged as potentially optimal, achieving CFU/g is less than 102  while maintaining relatively high GSH levels. Another approach showed the promise of adjusting pH changes to levels of 2.4-3.0 without altering inactivation conditions (95°C / 30 min) as an alternative to the previous one. Further studies are needed to explore the complex interplay between acidic conditions, duration of inactivation, and temperature parameters for simultaneously maximizing glutathione retention while controlling microbial viability.

glutathione; inactive dry yeasts; CFU
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