Еffect of downstream processing on content of glutathione and cell viability during production of inactive dry yeast
Glutathione (GSH) is a tripeptide thiol with important protective activity in living organisms. Due to its ability to prevent oxidation, this substance is commonly used in biotechnological products. The fed-batch process with yeasts of the genus Saccharomyces coupled with downstream processing to obtain inactive dry yeasts with high GSH content is the common method for industrial production. Short-term high-temperature treatment (1 minute at 105°C) emerged as potentially optimal, achieving CFU/g is less than 102 while maintaining relatively high GSH levels.