1. CTAS
  2. All Volumes and Issues
  3. Volume 8, Number 2
  4. Іmpact of nitrogen sources, salts, and В-group vitamins on the biosynthesis of lactic acid

Іmpact of nitrogen sources, salts, and В-group vitamins on the biosynthesis of lactic acid

CTAS.
2025;
Volume 8, Number 2
: 84-91
Authors:
  1. Dmytro Kiiv
  2. Sofiya Vasylyuk
1
Lviv Polytechnic National University, Ukraine
2
Department of Technology of Biologically Active Substances, Pharmacy & Biotechnology, Lviv National Polytechnic University

The study established a direct correlation between the concentration of corn steep liquor in the fermentation medium and the amount of lactic acid, as well as the biochemical requirements of Lactobacillus delbrueckii UY-2/13 for nitrogen-containing components of corn steep liquor. In particular, it was found that at a corn steep liquor concentration of 160 g/dm³, the maximum lactic acid concentration (101.37 g/dm³) was achieved with a yield of 94.39%. In addition, the effect of sodium acetate, ammonium citrate, Tween-80, vitamin B₂, manganese sulfate, and copper sulfate on lactic acid biosynthesis was investigated. These compounds increased the lactic acid concentration by 6.00, 3.62, 4.20, 3.61, 1.46, and 1.31 g/dm³, respectively. 

lactic acid
Lactobacillus delbrueckii
corn steep liquor
growth factors
B-group vitamins
metal ions
biosynthesis
  1. Wee, Y., Kim, J., & Ryu, H. (2006). Biotechnological production of lactic acid andits recent applications. Food Technology and Biotechnology, 44(2). Retrieved from https://www.researchgate.net/publication/228357901_Biotechnological_production_of_lactic_acid_and_its_recent_applications_Food_Technol.
  2. Abedi, E., & Hashemi, S. M. B. (2020). Lactic acid production - producing microorganisms and substrates sources-state of art. Heliyon, 6(10), e04974. https://doi.org/10.1016/j.heliyon.2020.e04974
  3. Kiiv, D., Vasylyuk, S., & Lubenets, V. (2024). Lactic acid: Industrial synthesis, microorganisms-producers and substrates: A review. Chemistry & Chemical Technology, 18(2), 157–169. https://doi.org/10.23939/chcht18.02.157.
  4. Saelee, N., & Sriroth, K. (2014). Optimization of nutrients in fermentative lactic acid production using oil palm trunk juice as substrate. Advances in Bioscience and Biotechnology, 05(12), 957–965. doi:10.4236/abb.2014.512109.
  5. Kiiv, D., & Vasylyuk, S. (2025). Effect of b vitamin addition on the biosynthesis of lactic acid by lactobacillus delbrueckii. Biotechnologia Acta, 18(2), 48–50. doi:10.15407/biotech18.02.048.
  6. Garrity, G., De Vos, P., Jones, D., Krieg, N., & Wolfgang, L. (2009). Bergey's manual of systematic bacteriology: Volume 3: The firmicutes (2nd edition). New York: Springer.
  7. Yoo, I.-K., Chang, H. N., Lee, E. G., Chang, Y. K., & Moon, S.-H. (1997). Effect of B vitamin supplementation on lactic acid production by Lactobacillus casei. Journal of Fermentation and Bioengineering, 84(2), 172–175. doi:10.1016/s0922-338x(97)82551-2.
  8. de la Torre, I., Ladero, M., & Santos, V. E. (2018). Production of d-lactic acid by Lactobacillus delbrueckii ssp. delbrueckii from orange peel waste: Techno-economical assessment of nitrogen sources. Applied Microbiology and Biotechnology, 102(24), 10511–10521. doi:10.1007/s00253-018-9432-4.
  9. Mawgoud, Y., Ibrahim, G., & El-ssayad, M. (2016). Studying the influence of nitrogen source on lactic acid production from whey permeate by immobilized Lactobacillus bulgaricus Lb-12. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 7(1). Retrieved from https://www.researchgate.net/publication/292464644_Studying_the_influence_of_nitrogen_source_on_lactic_acid_production_from_whey_permeate_by_immobilized_Lactobacillus_bulgaricus_Lb-12.
  10. Yu, L., Lei, T., Ren, X., Pei, X., & Feng, Y. (2008). Response surface optimization of l-(+)-lactic acid production using corn steep liquor as an alternative nitrogen source by Lactobacillus rhamnosus CGMCC 1466. Biochemical Engineering Journal, 39(3), 496–502. doi:10.1016/j.bej.2007.11.008.
  11. Téllez-Luis, S. J., Moldes, A. B., Vázquez, M., & Alonso, J. L. (2003). Alternative media for lactic acid production by lactobacillus delbrueckii NRRL B-445. Food and Bioproducts Processing, 81(3), 250–256. doi:10.1205/096030803322438018.
  12. Ye, T., Li, X., Zhang, T., Su, Y., Zhang, W., Li, J., ... Xue, G. (2018). Copper (II) addition to accelerate lactic acid production from co-fermentation of food waste and waste activated sludge: Understanding of the corresponding metabolisms, microbial community and predictive functional profiling. Waste Management, 76, 414–422. doi:10.1016/j.wasman.2018.03.028.
  13. Hayek, S. A., Gyawali, R., Aljaloud, S. O., Krastanov, A., & Ibrahim, S. A. (2019). Cultivation media for lactic acid bacteria used in dairy products. Journal of Dairy Research, 86(4), 490–502. doi:10.1017/S002202991900075X.
  14. Hayek, S. A., Shahbazi, A., Awaisheh, S. S., Shah, N. P., & Ibrahim, S. A. (2013). Sweet potatoes as a basic component in developing a medium for the cultivation of lactobacilli. Bioscience, Biotechnology, and Biochemistry, 77(11), 2248–2254. doi:10.1271/bbb.130508.
  15. Iino, T., Uchimura, T., & Komagata, K. (2002). The effect of sodium acetate on the growth yield, the production of L- and D-lactic acid, and the activity of some enzymes of the glycolytic pathway of Lactobacillus sakei NRIC 1071(T) and Lactobacillus plantarum NRIC 1067(T). The Journal of general and applied microbiology, 48(2), 91–102. https://doi.org/10.2323/jgam.48.91.
  16. Akermann, A., Weiermüller, J., Christmann, J., Guirande, L., Glaser, G., Knaus, A., & Ulber, R. (2020). Brewers’ spent grain liquor as a feedstock for lactate production with lactobacillus delbrueckii subsp. lactis. Engineering in Life Sciences, 20(5-6), 168–180. doi:10.1002/elsc.201900143.
  17. Coelho, L. F., Lima, C. J. B. d., Rodovalho, C. M., Bernardo, M. P., & Contiero, J. (2011). Lactic acid production by new Lactobacillus plantarum LMISM6 grown in molasses: Optimization of medium composition. Brazilian Journal of Chemical Engineering, 28(1), 27–36. doi:10.1590/s0104-66322011000100004.
  18. Nancib, A., Nancib, N., Meziane-Cherif, D., Boubendir, A., Fick, M., & Boudrant, J. (2005). Joint effect of nitrogen sources and B vitamin supplementation of date juice on lactic acid production by Lactobacillus casei subsp. rhamnosus. Bioresource technology, 96(1), 63–67. https://doi.org/10.1016/j.biortech.2003.09.018.
  19. Xu, G.-Q., Chu, J., Zhuang, Y.-P., Wang, Y.-H., & Zhang, S.-L. (2008). Effects of vitamins on the lactic acid biosynthesis of Lactobacillus paracasei NERCB 0401. Biochemical Engineering Journal, 38(2), 189–197. doi:10.1016/j.bej.2007.07.003.
  20. Ma, K., Cui, Y., Zhao, K., Yang, Y., Wang, Y., Hu, G., & He, M. (2022). D-Lactic acid production from agricultural residues by membrane integrated continuous fermentation coupled with B vitamin supplementation. Biotechnology for Biofuels and Bioproducts, 15(1). doi:10.1186/s13068-022-02124-y.
  21. Klotz, S., Kuenz, A., & Prüße, U. (2017). Nutritional requirements and the impact of yeast extract on the d-lactic acid production by Sporolactobacillus inulinus. Green Chemistry, 19(19), 4633–4641. doi:10.1039/c7gc01796k.