DPPH method

This work presents a study of the effectiveness of biosurfactant from the culture liquid of Bacillus subtilis sp. on the extraction of biologically active substances in the extraction of thyme Thymus serpyllum L. Ethanol extracts of plant material were obtained by maceration with the addition of biosurfactant in the amount of 0.01% to 0.1% and a corresponding increase in the yield of extractive substances was recorded by the gravimetric method in the range from 2 to 12 %.

The antioxidant properties of ethanol-water extracts of Scorzonera purpurea subsp. rosea obtained by maceration with ethanol content of 50 % (E50) and 70 % (E70) were studied using the DPPH (E50 – 82 %, E70 – 86 %), ABTS (E50 – 89 %, E70 – 93 %) and FRAP (E50 - 23.01 μmol/ml, E70 - 23.58 μmol/ml) methods. It was determined that the studied extracts differ little in the total content of extractive substances (E50 - 19.86 mg/ml, E70 - 19.4 mg/ml), the sum of polyphenolic compounds (E50 - 2.02 mgGC/ml, E70 - 2.11 mgGC/ml), flavonoids (E50 - 0.235 mgC/ml, E70 - 0.276 mgC/ml).