The antioxidant properties of ethanol-water extracts of Scorzonera purpurea subsp. rosea obtained by maceration with ethanol content of 50 % (E50) and 70 % (E70) were studied using the DPPH (E50 – 82 %, E70 – 86 %), ABTS (E50 – 89 %, E70 – 93 %) and FRAP (E50 - 23.01 μmol/ml, E70 - 23.58 μmol/ml) methods. It was determined that the studied extracts differ little in the total content of extractive substances (E50 - 19.86 mg/ml, E70 - 19.4 mg/ml), the sum of polyphenolic compounds (E50 - 2.02 mgGC/ml, E70 - 2.11 mgGC/ml), flavonoids (E50 - 0.235 mgC/ml, E70 - 0.276 mgC/ml). The obtained results indicate the expediency of using an alcohol-water mixture with an ethanol content of 50 % as a cheaper extractant for the preparation of Scorzonera purpurea extracts with a pronounced antioxidant effect.
1. Cherska, M., Kukharchuk, K., & Haiova, O. (2021). Oxidative stress in patients with high cardiovascular risk. Endokrynologia, 26(3), 287-297. https://doi.org/10.31793/1680-1466.2021.26-3.287
https://doi.org/10.31793/1680-1466.2021.26-3.287
2. Trokhymovych, A. A., Kyshko, M. M., Slyvka, YA. I., & Hanych, O. T. (2011). Vilʹnoradykalʹne okyslennya i antyoksydantna systema v sertsevo-sudynniy patolohiyi. Naukovyy visnyk Uzhhorodsʹkoho universytetu. Seriya "Medytsyna", 2 (41), 361-364.
3. .Borzykh, O.A., Lavrenko, A.V., Selikhova, L.H., Dihtyar, N.I., Herasymenko, ND., Avramenko, YA.M., Byelan, O.V., Mormolʹ, I.A., Kolomiyetsʹ, H.O., & Kaydashev, I.P. (2020). Suchasni aspekty vilʹnoradykalʹnoyi patolohiyi (ohlyad literatury ta vlasni doslidzhennya). Visnyk Ukrayinsʹkoyi medychnoyi stomatolohichnoyi akademiyi, 20(1), 4-8. DOI: 10.31718/2077-1096.20.1.4
https://doi.org/10.31718/2077-1096.20.1.4
4. Seredyuk, K. M., Stadnytsʹka, N. YE., Yaremkevych, O. S., Pavlyuk, I. V., & Dyakon, I. V. (2016). Doslidzhennya antyoksydantnoyi aktyvnosti ekstraktiv likarsʹkykh roslyn. Visnyk Natsionalʹnoho universytetu "Lʹvivsʹka politekhnika". Seriya: Khimiya, tekhnolohiya rechovyn ta yikh zastosuvannya, (841), 228-232.
5. Pavlyuk, I. V., Stadnytsʹka, N. YE., Yasitska-Misyak, I., Vechorek, P., Zahoriy, H. V., Brezvyn, O. M., Rudyk, H. V., & Novikov, V. P. (2015). Doslidzhennya biolohichnoyi aktyvnosti vtorynnoho ekstraktu zi shrotu travy materynky zvychaynoyi (Origanum vulgare). Ukrayinsʹkyy biofarmatsevtychnyy zhurnal, 1(36), 21-24.
6. Pavlyuk, I., Stadnytska, N., Jasicka-Misiak, I., Gorka, B., Wieczorek, P. P., & Novikov, V. (2015). A study of the chemical composition and biological activity of extracts from wild carrot (Daucus carota L.) seeds waste. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(2), 603-611. http://www.rjpbcs.com/pdf/2015_6(2)/[91].pdf
7. 7.Stadnytsʹka, N. YE., Dyakon, I. V., Bolibrukh, L. D., Misyk, YA. T., Hubytsʹka, I. I., & Novikov, V. P. (2018). Nastoyanka pervotsvitu vesnyanoho - dzherelo fenolʹnykh spoluk z antyoksydantnoyu aktyvnistyu. Visnyk Natsionalʹnoho universytetu «Lʹvivsʹka politekhnika». Khimiya, tekhnolohiya rechovyn ta yikh zastosuvannya, 1(1), 94-98.
https://doi.org/10.23939/ctas2018.01.094
8. 8.Stadnytsʹka, N. YE., Pavlyuk, I. V., Shvetsʹ, V. V., Hubytsʹka, I. I., Lubenetsʹ, V. I., & Novikov, V. P. (2012). Vybir optymalʹnykh tekhnolohichnykh parametriv otrymannya antymikrobnoyi nastoyanky. Visnyk Natsionalʹnoho universytetu «Lʹvivsʹka politekhnika». Khimiya, tekhnolohiya rechovyn ta yikh zastosuvannya, (726), 105-110.
9. Xie, Y., Guo, Q-S., & Wang, G-S. (2016). Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins. Molecules, 21, 768. DOI: 10.3390/molecules21060768
https://doi.org/10.3390/molecules21060768
10. Ugur, A., Sarac, N., Ceylan, O., Duru, M. E., & Beyatli, Y. (2010). Chemical composition of endemic Scorzonera sandrasica and studies on the antimicrobial activity against multiresistant bacteria. Journal of Medicinal Food, 13(3), 635-639. DOI: 10.1089/jmf.2008.0312
https://doi.org/10.1089/jmf.2008.0312
11. Yang, Y-J., Liu, X., Wu, H-R., He, X-F., Bi, Y-R., Zhu, Y., et al. (2013). Radical scavenging activity and cytotoxicity of active quinic acid derivatives from Scorzonera divaricata roots. Food Chemistry, 138, 2057-2063. DOI: 10.1016/j.foodchem.2012.10.122
https://doi.org/10.1016/j.foodchem.2012.10.122
12. Wu, Q-X., Su, Y-B., & Zhu, Y. (2011). Triterpenes and steroids from the roots of Scorzonera austriaca. Fitoterapia, 82, 493-496. DOI: 10.1016/j.fitote.2011.01.006
https://doi.org/10.1016/j.fitote.2011.01.006
13. Akkol, E. K., Šmejkal, K., Kurtul, I., Ilhan, M., Güragac, F. T., İşcan, G. S., et al. (2019). Inhibitory activity of Scorzonera latifolia and its components on enzymes connected with healing process. Journal of Ethnopharmacology, 245, 112168. DOI: 10.1016/j.jep.2012.01.015
https://doi.org/10.1016/j.jep.2012.01.015
14. Acıkara, O. B., Özbilgin, S., Saltan-̇Iscan, G., Dall'Acqua, S., Rjašková, V., Özgökçe, F., et al. (2018). Phytochemical Analysis of Podospermum and Scorzonera n-Hexane Extracts and the HPLC Quantitation of Triterpenes. Molecules, 23, 1813.DOI: 10.3390/molecules23071813
https://doi.org/10.3390/molecules23071813
15. Sahina, H., Sarı, A., Ozsoy, N., Çelik, B. O., & Koyuncu, O. (2018). Two new phenolic compounds and some biological activities of Scorzonera pygmaea Sibth. & Sm. subaerial parts. Natural product research, 34(5), 621-628. https://doi.org/10.1080/14786419.2018.1493585
https://doi.org/10.1080/14786419.2018.1493585
16. Granica, S., Lohwasser, U., Jöhrer, K., & Zidorn, C. (2015). Qualitative and quantitative analyses of secondary metabolites in aerial and subaerial of Scorzonera hispanica L. (black salsify). Food Chemistry, 173, 321-331. DOI: 10.1016/j.foodchem.2014.10.006
https://doi.org/10.1016/j.foodchem.2014.10.006
17. Akkol, E. K., Acıkara, O. B., Süntar, I., Ergene, B., & Çitoglu, G. S. (2012). Ethnopharmacological evaluation of some Scorzonera species: In vivo anti-inflammatory and antinociceptive effects. Journal of Ethnopharmacology, 140, 261-270. https://doi.org/10.1016/j.jep.2012.01.015
https://doi.org/10.1016/j.jep.2012.01.015
18. Akkol, E. K., Acıkara, O. B., Süntar, I., Citoglu, G. S., Keles, H., & Ergene, B. (2011). Enhancement of wound healing by topical application of Scorzonera species: Determination of the constituents by HPLC with new validated reverse phase method. Journal of Ethnopharmacology, 137, 1018-1027. DOI: 10.1016/j.jep.2011.07.029
https://doi.org/10.1016/j.jep.2011.07.029
19. Wu, Q.-X., He, X.-F., Jiang, C.-X., Zhang, W., Shi, Z.-N., Li, H.-F., & інші (2018). Two novel bioactive sulfated guaiane sesquiterpenoid salt alkaloids from the aerial parts of Scorzonera divaricata. Fitoterapia, 124, 113-119. https://doi.org/10.1016/j.fitote.2017.10.011
https://doi.org/10.1016/j.fitote.2017.10.011
20. Acikara, O. B., Hošek, J., Babula, P., Cvacka, J., Budešínský, M., Dracinský, M., & інші (2016). Turkish Scorzoneraspecies extracts attenuate cytokine secretion via inhibition of NF-κB activation, showing anti-inflammatory effect in vitro. Molecules, 21(43), 1-14. https://doi.org/10.3390/molecules21010043
https://doi.org/10.3390/molecules21010043
21. Bahadır, Ö., Citoglu, G. S., Smejkal, K., Dall'Acqua, S., Özbek, H., Cvacka, J., & інші (2010). Analgesic compounds from Scorzonera latifolia (Fisch. and Mey.) DC. Journal of Ethnopharmacology, 131, 83-87. https://doi.org/10.1016/j.jep.2010.06.003
https://doi.org/10.1016/j.jep.2010.06.003
22. Sweidan, A., El-Mestrah, M., Kanaan, H., & Dandache, I. (2020). Antibacterial and antibiofilm activities of Scorzonera mackmeliana. Pak. J. Pharm. Sci., 33(1), 199-206.
23. Athmouni, K., Belghith, T., Bellassouad, K., & Ayadi, A. H. (2015). Effect of extraction solvents on the biomolecules and antioxidant properties of Scorsonera undulata (Asteraceae): application of factorial design optimisation phenolic extraction. Acta Sci. Pol. Technol. Aliment, 14(4), 313-330. DOI: 10.17306/J.AFS.2015.4.32.
https://doi.org/10.17306/J.AFS.2015.4.32
24. Nesteruk, Y. (2003). The plant world of the Ukrainian Carpathians: Chornohora: ecological travels: reference book. Lviv, Ukraine, 520 с.
25. Jasicka-Misiak, I., Makowicz, E., & Stanek, N. (2021). Chromatographic fingerprint, antioxidant activity, and colour characteristic of Polish goldenrod (Solidago virgaurea L.) honey and flower. European Food Research and Technology, 244, 1169-1184.
https://doi.org/10.1007/s00217-018-3034-3
26. Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., & Ju, Y.-H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal, 22(3), 296-302. doi: 10.1016/j.jfda.2013.11.001. Epub 2013 Dec 18.
https://doi.org/10.1016/j.jfda.2013.11.001
27. Katalinic, V., Milos, M., Kulisic, T., & Jukic, M. (2006). Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry, 94(4), 550-557. https://doi.org/10.1016/j.foodchem.2004.12.004
https://doi.org/10.1016/j.foodchem.2004.12.004