: 63-68
National University Lviv Polytechnic
Lviv Polytechnic National University
Lviv Polytechnic National University
Lviv Polytechnic National University
Lviv Polytechnic National University

The callus biomass of Calendula officinalis was obtained in vitro by using the
biotechnological method. The results of the chromatography-mass-spectrometry analysis for
the content of biologically active substances (BAS), obtained from plant chloroform extracts:
from seeds (identified 5 compounds) and callus biomass (identified 11 compounds) are shown.
Biological screening of BAS from callus biomass was conducted. The production of callus
biomass and biologically active chloroform extract which is obtained on its basis are an
alternative sources of Calendula officinalis raw materials, which are used for the
pharmaceutical and cosmetic industry.

1. Direct resistively heatedcolumn gas chromatography (Ultrafast module-GC) for high-speed
analysis of essential oils of differing complexities / C. Bicchi, C. Brunelli, C. Cordero [etc.] //
J. Chromatogr. A. – 2004. – Vol. 1024, No. 1–2. – Р. 195–207. 7. A consumer’s dictionary of cosmetic
ingredients / Ryth Winter. – 7th ed. – New York, 2009– Р.565. 2. Phytochemical Constituents and
Pharmacological Activities of Calendula officinalis Linn (Asteraceae): A Review / B. P. Muley,
S. S. Khadabadi, N.B. Banarase // Tropical Journal of Pharmaceutical Research – 2009. – Vol. 8 (5) –
Р. 455–465. 3. Zitterl-Eglseer K., Sosa S., Jurenitsch J., Schubert-Zsilavecz M., Della Loggia R., Tubaro
A., Bertoldi M., Franz C. Anti-oedematous activities of the main triterpendiol esters of marigold
(Calendula officinalis L.) // Journal Ethnopharmacol. – Vol. 57(2). – 1997. – P. 139–44.4. Jiménez-
Medina E., Garcia-Lora A., Paco L., Algarra I., Collado A., Garrido F. A new extract of the plant
Calendula officinalis produces a dual in vitro effect:cytotoxic anti-tumor activity and lymphocyte
activation // BMC Cancer. – Vol. 5(6). – 2006. – P. 119. 5. S. Gadzovska, V. Pavlova, M. Nasteska,
A. Neskoska, M. Spasenoski. . Secondary metabolite production in in vitro shoots of marigold (Calendula
officinalis L.) / Proceedings of the III Congress of Ecologists of Macedonia. P. 54–61. 6. Hashim K.,
Mohammed Al-oubaidi, Aseel Salih Mohammed Ameen / Increasing secondary metabolites of Calendula
officinalis L. using salicylic acid in vitro / World Journal of Pharmacy and Pharmaceutical Sciences,
Vol. 3, Issue 5, 2014. P. 1146–1155.