OBTAINING AND INVASTIGATION EXTRACTS OF CALLUS BIOMASS OF САLENDULA OFFICINALIS

2018;
: 63-68
1
National University Lviv Polytechnic
2
Lviv Polytechnic National University
3
Lviv Polytechnic National University
4
Lviv Polytechnic National University
5
Lviv Polytechnic National University

Modern biotechnological methods for plant biomass production have relevance, since the use of medicinal plants is connected with a limited resource potential of many valuable species, with germination in polluted environment, using plants that are on the limit of destruction or listed in the Red Book. The aim of the work was to obtain the Calendula officinaliscallus biomass by cell culture and tissue culture methods, the research of the component composition of chloroform extracts from wild-type seeds and Calendula officinalis callus biomass for the purpose of integrated use of plant raw material. For research purposes were used seeds of calendula (Calendula officinalis L.), which were collected in Lviv region. A part of the seeds was cultivated invitro, where they were previously stratified in a solution of 0.01 g/l of gibberellinic acid, and sterilized with 96% ethanol and 30% hydrogen peroxide solution. Aseptic explants were cultivated on an agar medium with 2,4-D growth regulators and kinetin for 50 days. Cultures were maintained in a laminar box under fluorescent lamps with illumination of 2000 lux, with daily photoperiod of 16 hours at 25 ± 2 ° С. The amount of 40 g Calendula officinalis callus biomass was obtained. Extracts from seeds and Calendula officinalis biomass were obtained by maceration with duration of 2 days; extractor - 90% chloroform (CHCl3). The component composition of the biologically active substances (BAS) of the extracts that wasobtained, laterhas been determined by chromatography using a spectrometric method. Among the BASs contained in the extract of Calendula officinalis seeds, 5 compounds were identified by the gas-liquid chromatography method, and they were introduced by carbohydrates (eicosan, heptacosan, hexacosan, (Z) -7-hexadecenylacetate) and fatty acids (palmitic acid).Substances from chloroform extracts of Calendula officinalis callus biomass were identified on chromatogram, and they were introduced by 11 carbohydrate compounds (octacosan, eicosan, oxiran, gentriacontan, 1,19-eicosadine, (Z) -7-hexadecenylacetate), fatty acids (palmitic, arachidonic, octadecanoic, trichloroacetic acid), and phytosterols (behenyl alcohol).

1. Direct resistively heatedcolumn gas chromatography (Ultrafast module-GC) for high-speed
analysis of essential oils of differing complexities / C. Bicchi, C. Brunelli, C. Cordero [etc.] //
J. Chromatogr. A. – 2004. – Vol. 1024, No. 1–2. – Р. 195–207. 7. A consumer’s dictionary of cosmetic
ingredients / Ryth Winter. – 7th ed. – New York, 2009– Р.565. 2. Phytochemical Constituents and
Pharmacological Activities of Calendula officinalis Linn (Asteraceae): A Review / B. P. Muley,
S. S. Khadabadi, N.B. Banarase // Tropical Journal of Pharmaceutical Research – 2009. – Vol. 8 (5) –
Р. 455–465. 3. Zitterl-Eglseer K., Sosa S., Jurenitsch J., Schubert-Zsilavecz M., Della Loggia R., Tubaro
A., Bertoldi M., Franz C. Anti-oedematous activities of the main triterpendiol esters of marigold
(Calendula officinalis L.) // Journal Ethnopharmacol. – Vol. 57(2). – 1997. – P. 139–44.4. Jiménez-
Medina E., Garcia-Lora A., Paco L., Algarra I., Collado A., Garrido F. A new extract of the plant
Calendula officinalis produces a dual in vitro effect:cytotoxic anti-tumor activity and lymphocyte
activation // BMC Cancer. – Vol. 5(6). – 2006. – P. 119. 5. S. Gadzovska, V. Pavlova, M. Nasteska,
A. Neskoska, M. Spasenoski. . Secondary metabolite production in in vitro shoots of marigold (Calendula
officinalis L.) / Proceedings of the III Congress of Ecologists of Macedonia. P. 54–61. 6. Hashim K.,
Mohammed Al-oubaidi, Aseel Salih Mohammed Ameen / Increasing secondary metabolites of Calendula
officinalis L. using salicylic acid in vitro / World Journal of Pharmacy and Pharmaceutical Sciences,
Vol. 3, Issue 5, 2014. P. 1146–1155.