Bacteria belonging to the genus Bacillus produce biogenic surface-active compounds which are a sustainable alternative to synthetic ones. The amount of the target products depends significantly on the conditions of microorganism cultivation, especially the components of the medium. In this study, we utilized Bacillus spp. strain that can grow in a glycerol-containing medium. We used the Monod model to determine the growth kinetics of the bacteria depending on the concentration of nutrients in the medium. Our findings indicated that intensive growth of Bacillus spp. bacteria is observed during cultivation in the medium with a glycerol concentration of 30-40 g/L. The surface-active substances from Bacillus spp. was characterized by a sufficient level of foaming and foam stability. The highest value of foam stability was observed on the 7th day of cultivation.
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