Study of antioxidant properties and component composition of cereal crop extracts

1
Lviv Polytechnic National University
2
Lviv Polytechnic National University
3
Lviv Polytechnic National University
4
Lviv Polytechnic National University

The scientific work researched the level of inhibition of Fenton's reagent-initiated lipid peroxidation (LPO) and oxidative modification of proteins (OMP) processes in rat hepatocytes in vitro, as an effect of aqueous, and 40, 70% aqueous-ethanolic extracts of wheat and oat bran. Qualitative reactions were used to assess the total amount of phenolic compounds, especially flavonoids. Thin-layer chromatography (TLC) identified gallic acid, ferulic acid, and quercetin in the samples of the studied objects. The study results confirm the presence of phenolic compounds, flavonoids, and amino acids in the resulting bran extracts. Spectrophotometric analysis revealed a relatively low level of antioxidant activity of the studied extracts.

  1. Reddy, S.S., & Krishnan, C. (2010). Production of prebiotics and antioxidants as health food supplements from lignocellulosic materials using multienzymatic hydrolysis. International Journal of Chemical Sciences, 3(3), 535–549.
  2. Kaprelyants, L., & Zhurlova, O. (2017). Technology of wheat and rye bran biotransformation into functional ingredients. International Food Research Journal, 24(5), 1975–1979.
  3. Яремкевич, О.С., & Федоришин, О.М. (2024). Дослідження антиоксидантних властивостей екстрактів відкасника безстеблового (Carlina acaulis L.), арніки гірської (Arnica montana L.) та календули лікарської (Calendula officinalis L.). Chemistry, Technology and Application of Substances, 7(1), 103-111.
  4. Strilchuk, L. M. (2020). Malondialdehyde and its role in pathogenesis of unstable forms of ischemic heart disease. Medicine Today and Tomorrow, 87(2), 20–25. https://doi.org/10.35339/msz.2020.87.02.02
  5. Pengkumsri, N., Chaiyasut, C., Sivamaruthi, B. S., Saenjum, Ch., Sirilun, S., Peerajan, S.,  Suwannaler, T. P., Sirisattha, S., Chaiyasut, Kh., & Kesika, P. (2015). The influence of extraction methods on composition and antioxidant properties of rice bran oil. Food Science and Technology. 35(3), 493–501. https://doi.org/10.1590/1678-457x.6730
  6. Dewi, S., Sadikin, M., & Mulyawan, W. (2021). Oxidative stress in the heart of rats exposed to acute intermittent hypobaric hypoxia. The Ukrainian Biochemical Journal, 93(3), 68–74. https://doi.org/10.15407/ubj93.03.068
  7. Vitalini, S., Beretta, G., Iriti, M., Orsenigo, S., Basilico, N., Dall’Acqua, S., Iorizzi, M., & Fico, G. (2011). Phenolic compounds from Achillea millefolium L. and their bioactivity. Acta Biochim. Pol., 58(2), 203-219.
  8. Pavlyuk, I., Stadnytska, N., Jasicka-Misiak, I., Gorka, B., Wieczorek, P. P., & Novikov, V. (2015). A study of the Chemical Composition and Biological Activity of Extracts from Wild Carrot (Daucus carota L.) Seeds Waste. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(2), 603-611.
  9. Jasicka-Misiak, I., & Stanek, N. (2018). HPTLC Phenolic Profiles as Useful Tools for the Authentication of Honey. Food Analytical Methods, 11, 2979–2989.
  10. Quy, D. D., Artik, E. A., Phuong, L. T.-N., & Lien, H. H. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis. 2014. Vol. 22, No. 3. P. 296–302. https://doi.org/10.1016/j.jfda.2013.11.001
  11. Blyznyuk, N., Prokopenko, Yu., & Georgiyants, V. (2016). Development of methods for determination of phenolic acids and flavonoids in capsules containing Corylus avellana L. dry extract. ScienceRise: Pharmaceutical Science, 2(4), 18–22.
  12. Karpiuk, V., & Konechna, R. (2021). Total phenolic and flavonoid content, antioxidant activity of Ficarea verna. Scientific journal of Polonia university, 46(3), 229-234.
  13. Zarivna, N.O. (2014). Study of the amino acid composition of the herb Thymus Serpyllum. Ukrainian Biopharmaceutical Journal, 3 (32), 67-70.
  14. Лущак, В.I., Багнюкава, T.В., & Лужна, Л.I. (2006). Показники оксидативного стресу. 2. Перекиси ліпідів.Український біохімічний журнал, 78(5), 113–119.
  15. Morgan, G. A., Leech, N. L., Gloeckner, G. W., & Barrett, K. C. (2012). IBM SPSS for Introductory statistics. In: Use and Interpretation, 4-th ed.; Routledge Taylor & amp; Francis Group, New York.