Obtaining biomass of echinacea purpurea in vitro
This work investigated the possibility of introducing Echinacea purpurea into in vitro culture in order to obtain callus biomass. The method of seed sterilization and seedling cultivation on Murashige-Skoog medium was described. The effect of various combinations of growth regulators (IAA, BAP, NAA, 2,4-D) on callus formation from leaf, stem and root explants was experimentally determined. It was found that the formation of callus biomass occurs better on a medium with the addition of growth regulators IAA and BAP in a ratio of 2:1 mg/l than NAA, 2,4-D in a ratio of 1:1 mg/l.