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  3. Volume 17, Number 1, 2023
  4. Characterization, Antioxidant Activity, and In Silico Molecular Docking of Chitosan from Snail Shell Waste by Ultrasonic Technique

Characterization, Antioxidant Activity, and In Silico Molecular Docking of Chitosan from Snail Shell Waste by Ultrasonic Technique

JCCT.
2023;
Volume 17, Number 1
: pp. 126 - 132
https://doi.org/10.23939/chcht17.01.126
Authors:
  1. Umarudin
  2. Sri Rahayu
  3. Sri Widyarti
  4. Warsito
1
Doctoral student of Biology, Faculty of Mathematic and Natural Sciences, University of Brawijaya, Department of Pharmacy, Diploma III Pharmacy, Academy Pharmacy of Surabaya
2
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya
3
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Brawijaya
4
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Essential Oil's Institute, University of BrawijayaUniversity of Brawijaya,

Snails are often found in Indonesia, especially at Kediri, but the snail shell has no commercial value. This research report describes the characterization and antioxidant activity of chitosan from snail shell waste (chitosan-SSW) by ultrasonic technique and analyzes the potential of chitosan as an inhibitor of receptors of free radicals using an in silico molecular docking method. Characterization of chitosan-SSW was performed to analyze the content of water, protein, and functional groups as well as molecular weight, particle size, mor-phology, antioxidant activity, and in silico molecular docking. We found that chitosan-SSW with ultrasonic treatment had a high degree of deacetylation (DD) and high molecular weight (MW). The characteristic of chitosan-SSW was found to be as follows: water content of 0.43 %, protein content of 1.59 %, molecular weight of 2.198 kDa, and deacetylation degree value of 79.50 %. Importantly, chitosan-SSW had high antioxidant activity to potentially reduce free radical of DPPH with IC50 value of 2.44 µg/mL. Chitosan is predicted to have the potential as an inhibitor of lipoxygenase, CYP2C9, and NADPH-oxidase.

chitosan-SSW
characterization
ultrasound
antioxidant
in silico molecular docking
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