: 21-27
Lviv Polytechnic National University, Department of Hydraulic and Sanitary Engineering
Lviv Polytechnic National University
Lviv Polytechnic National University, Department of Hydraulic and Water Engineering

Wastewater from human settlements contains a significant amount of organic and biogenic substances. Insufficiently treated wastewater enters surface water and leads to their eutrophication. The usage of microalgae in wastewater treatment has significant advantages in comparison with other methods of removing biogenic substances. Namely: effective and simultaneous removal of nitrogen and phosphorus without reagents management facilities, oxygen formation. Using microalgae in wastewater treatment is a new environmentally friendly biotechnological method. Microalgae grow well in wastewater, from which they absorb pollutants. The purpose of the study is to analyze the work and determine the possibility of intensification of sewage treatment plants in the western region of Ukraine with a population of about 18,900 inhabitants. Productivity of treatment plant is 3400 m3/day. Experimental investigation consisted in adding a concentrate of a living microalgae strain of the species Chlorella vulgaris to the wastewater that was entered to the treatment plant during May-September 2019. During the research, the results of wastewater analyzes conducted by the chemical laboratory of the municipal water supply and sewerage company were used. The results of the survey and analysis of the city's treatment plant indicate an insufficient degree of wastewater treatment. The effectiveness of Chlorella vulgaris at the treatment plant has been experimentally proven. Mathematical dependences of the effect of wastewater treatment (using Chlorella vulgaris) on their temperature according to the indicators: BOD5, COD, concentration of ammonium nitrogen, phosphates and suspended solids were obtained. Dependencies are described by a linear function that characterizes the general behavior of the obtained data. The obtained results made it possible to significantly reduce the negative impact of treatment plants on the environment.

Beuckels, A., Smolders, E., Muylaert, K. (2014) Nitrogen availability influences phosphorus removal in microalgae-based wastewater treatment. Water Research, 77, 98-106.

Gómez-Guzmán, A., Jiménez-Magaña, S., Guerra-Rentería, A.S., Gómez-Hermosillo, C., Parra-Rodríguez, F.J., Velázquez, S., Aguilar-Uscanga, B.R., Solis-Pacheco, J., González-Reynoso, O.: Evaluation of nutrients removal (NO3-N, NH3-N and PO4-P) with Chlorella vulgaris, Pseudomonas putida, Bacillus cereus and a consortium of these microorganisms in the treatment of wastewater effluents. Water Sci. Technol., 76, 49-56 (2017).

Guerra-Renteria, A. S., García-Ramírez, M. A., Gómez-Hermosillo, C., Gómez-Guzmán, A., González-García, Y., & González-Reynoso, O. (2019) Metabolic Pathway Analysis of Nitrogen and Phosphorus Uptake by the Consortium between C. Vulgaris and P. aeruginosa. International journal of molecular sciences, 20(8), 1978.

Abdel-Raouf, N., Al-Homaidan, A.A., Ibraheem, I.B.M. (2012) Microalgae and wastewater treatment. Saudi J. Biol. Sci., 19(3), 257-275.

Molazadeh, M., Ahmadzadeh, H., Pourianfar, H. R., Lyon, S., Rampelotto, P. H.: The Use of Microalgae for Coupling Wastewater Treatment With CO2 Biofixation. Frontiers in bioengineering and biotechnology, 7, 42 (2019).

Mayhead, E., Silkina, A., Llewellyn, C. A., Fuentes-Grünewald, C. (2018) Comparing Nutrient Removal from Membrane Filtered and Unfiltered Domestic Wastewater Using Chlorella vulgaris. Biology, 7(1), 12.

Amenorfenyo, D. K., Huang, X., Zhang, Y., Zeng, Q., Zhang, N., Ren, J., Huang, Q. (2019) Microalgae Brewery Wastewater Treatment: Potentials, Benefits and the Challenges. International journal of environmental research and public health, 16(11), 1910.

Szwarc, K., Szwarc, D., Zieliński, M. (2020) Removal of biogenic compounds from the post-fermentation effluent in a culture of Chlorella vulgaris. Environmental science and pollution research international, 27(1), 111-117.

Khalekuzzaman, M., Alamgir, M., Islam, M. B., Hasan, M. (2019) A simplistic approach of algal biofuels production from wastewater using a Hybrid Anaerobic Baffled Reactor and Photobioreactor (HABR-PBR) System. PloS one, 14(12), e0225458.

Pereira, S.F.L., Gonçalves, A.L., Moreira, F.C., Silva, T.F.C.V., Vilar, V.J.P., Pires, J.C.M. (2016) Nitrogen Removal from Landfill Leachate by Microalgae. Int. J. Mol. Sci., 17(11), 1926.

Muylaert, K., Beuckels, A., Depraetere, O., Foubert, I., Markou, G, Vandamme, D. (2015) Wastewater as a Source of Nutrients for Microalgae Biomass Production. In.: Moheimani, N.R., McHenry, M.P., de Boer, K., Bahri, P. (Eds.). Biomass and Biofuels from Microalgae: Advances in Engineering and Biology. Springer, Cham, pp 75-94.

Wang, Z., Gao, M., Wei, J., M,a K., Zhang, J., Yang, Y., Yu, S. (2016) Extracellular polymeric substances, microbial activity and microbial community of biofilm and suspended sludge at different divalent cadmium
concentrations. Bioresour. Technol., 205, 213-221.

Wang, J. H., Zhang, T. Y., Dao, G. H., Xu, X. Q., Wang, X. X., Hu, H. Y. (2017) Microalgae-based advanced municipal wastewater treatment for reuse in water bodies. Applied microbiology and biotechnology,
101(7), 2659-2675 (2017).

Renuka, N., Sood, A., Prasanna, R., Ahluwalia, A.S. (2015) Phycoremediation of wastewaters: a synergistic approach using microalgae for bioremediation and biomass generation. Int. J. Environ. Sci. Technol., 12, 1443-1460.

Borowitzka, M.A. (2013) Energy from Microalgae: A Short History. In: Borowitzka, M., Moheimani, N. (eds.) Algae for Biofuels and Energy. Developments in Applied Phycology, vol 5. Springer, Dordrecht.

Bhatt, N. C., Panwar, A., Bisht, T. S., Tamta, S. (2014) Coupling of algal biofuel production with wastewater. The Scientific World Journal, 2014, Article ID 210504.

Olguín EJ. (2012) Dual purpose microalgae-bacteria-based systems that treat wastewater and produce biodiesel and chemical products within a biorefinery. Biotechnol Adv., 30(5), 1031‐10462012.