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  2. All Volumes and Issues
  3. Volume 12, Number 2, 2018
  4. Hydrotrope-Induced Micellar Phase of Sodium Dodecyl Slfate as New Detergent for Extraction and Stabilization of Proteins

Hydrotrope-Induced Micellar Phase of Sodium Dodecyl Slfate as New Detergent for Extraction and Stabilization of Proteins

JCCT.
2018;
Volume 12, Number 2
: pp. 196-201
https://doi.org/10.23939/chcht12.02.196
Authors:
  1. Viktoriia Starova
  2. Sergey Kulichenko
1
Taras Shevchenko National University of Kyiv
2
Taras Shevchenko National University of Kyiv

X-ray phase analysis and IR spectroscopy data have confirmed a gel-like nature of sodium dodecyl sulfate micellar phase induced by sodium chloride and salicylic acid additives. This phase essentially consists of water (ωH2O > 50 %), which is connected with main components of the micellar-extraction system through intermolecular hydrogen bonds. Capacity of such micellar phase to quantitatively extract of proteins (R > 94 %) with saving of their catalytic activities and dispersity was found.

micellar extraction
sodium dodecyl sulfate
protein
native state
catalytic activity

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