1. CTAS
  2. All Volumes and Issues
  3. Volume 6, Number 1
  4. Study of alcoholic-water extracts of Thymus Vulgaris obtained by ultrasonic extraction method

Study of alcoholic-water extracts of Thymus Vulgaris obtained by ultrasonic extraction method

CTAS.
2023;
, Number 1
: 83-87
https://doi.org/10.23939/ctas2023.01.083
Authors:
  1. A.O. Kyrychuk
  2. Yu. М. Semenchuk
  3. M. S. Kurka
  4. Nataliya Stadnytska
1
Lviv Polytechnic National University
2
Lviv Polytechnic National University
3
Lviv Polytechnic National University
4
Department of Technology of Biologically Active Substances, Pharmacy & Biotechnology, Lviv National Polytechnic

Alcohol-water extracts of Thymus vulgaris have obtained by ultrasonic extraction method for different times extraction. The obtained extracts have been tested for the quantitative content of extractive substances, phenolic compounds and flavonoids and for the presence of antioxidant activity. The best studied indicators were found in extract obtained by ultrasound extraction for 40 minutes (content of extractive substances 25 mg / ml, sum of polyphenolic compounds 30 mg / ml, flavonoids content 45 mg / ml).

polyphenolic compounds
flavonoids
antioxidant activity
DPPH
ABTS
FRAP
  1. Bryda O., Stadnytska N. (2021). Extraction methods of extractive substances from medicinal plant raw materials: advantages and limitations.Annals of the Romanian Society for Cell Biology. 25(5), 1737- 1751.
  2. Bryda O. R., Stadnytska N. Ye., Mylianych A. O., Maltyz I. S., Shalata V.Ia. (2020). Perevaha metodu ekstraktsii z postiinym rukhom ekstrahenta na prykladi oderzhannia ekstraktu korenia pelarhonii ochytkovoi (Pelargonium sidoides). Chemistry, Technology and Application of Substances = Khimiia, tekhnolohiia rechovyn ta yikh zastosuvannia.  3(1), 110-116. https://doi.org/10.23939/ctas2020.01.110
  3. Pavliuk I.V., Stadnytska N.Ye., Novikov V.P. (2015). Doslidzhennia kinetyky ekstrahuvannia flavonoidiv zi shrotu shyshok khmeliu. Skhidno-Yevropeiskyi zhurnal peredovykh tekhnolohii.  5/11(77), 36-41. https://doi.org/10.15587/1729-4061.2015.50965
  4. Boiko M.M., Zaitsev O.I. (2008). Vyvchennia vplyvu ultrazvuku na kinetyku vyluchennia biolohichno aktyvnykh rechovyn z roslynnoi syrovyny. Ukrainskyi zhurna klinichnoi ta laboratornoi medytsyny. 3(3), 53 - 55 [Ukraine].
  5. Sydorov Yu.I. (2008). Ekstraktsiia roslynnoi syrovyny / I.Iu. Sydorov, I.I. Hubytska, R.T. Konechna, V.P. Novikov.  Lviv Vydavnytstvo Natsionalnoho universytetu "Lvivska politekhnika", 334 s. [Ukraine].
  6. Pavliuk I.V., Stadnytska N.Ye. (2015). Optymizatsiia umov tekhnolohichnoho protsesu pererobky shrotu Origanum vulgare, Daucus сarota, Humulus lupulus. Visnyk Natsionalnoho universytetu «Lvivska politekhnika». Khimiia tekhnolohiia rechovyn ta yikh zastosuvannia. 812, 251-255. [Ukraine].
  7. Starchevsky V., Kislenko V., Maksymiv N., Oliynyk, L. (2014).Investigation of kinetics conformities with a law of decomposition of agglomerates of microorganisms in the condition of acoustic cavitation. Eastern-European Journal of Enterprise Technologies, 1(10), 8-11. https://doi.org/10.15587/1729-4061.2014.20037
  8. Bernatska, N., Starchevsky, V. (2014). Oxidation kinetics of organic disintegration products of yeast in cavitation conditions.Eastern-European Journal of Enterprise Technologies, 4(10),  63-66. https://doi.org/10.15587/1729-4061.2014.25943
  9. Dauqan, E. M. A. (2017). Medicinal and functional values of thyme (thymus vulgaris L.) herb. Journal of Applied Biology & Biotechnology. https://doi.org/10.7324/JABB.2017.50203
  10. Kwon, H. I., Jeong, N. H., Jun, S. H., Son, J. H., Kim, S., Jeon, H., Kang, S. C., Kim, S. H., & Lee, J. C. (2018). Thymol attenuates the worsening of atopic dermatitis induced by Staphylococcus aureus membrane vesicles. International Immunopharmacology, 59, 301-309. https://doi.org/10.1016/j.intimp.2018.04.027
  11. Pascoe Canada. (n.d.). Thyme. Pascoe Canada. Retrieved February 19, 2023, from https://www.pascoe.ca/herbal-index/thyme#howdoesitwork
  12. Kuete, V. (2017). Medicinal spices and vegetables from Africa: Therapeutic potential against metabolic, inflammatory, infectious and systemic diseases. Academic Press.
  13. Basch, E., Ulbricht, C., Hammerness, P., Bevins, A., & Sollars, D. (2004). Thyme (thymus vulgarisL.), Thymol. Journal of Herbal Pharmacotherapy, 4(1), 49-67. https://doi.org/10.1080/J157v04n01_07
  14. Čabarkapa, I., Čolović, R., Đuragić, O., Popović, S., Kokić, B., Milanov, D., & Pezo, L. (2019). Anti-biofilm activities of essential oils rich in carvacrol and thymol againstsalmonellaenteritidis. Biofouling, 35(3), 361-375. https://doi.org/10.1080/08927014.2019.1610169
  15. Diánez, F., Santos, M., Parra, C., Navarro, M. J., Blanco, R., amp; Gea, F. J. (2018). Screening of antifungal activity of 12 essential oils against eight pathogenic fungi of vegetables and mushroom. Letters in Applied Microbiology, 67(4), 400-410. https://doi.org/10.1111/lam.13053
  16. Gucwa, K., Milewski, S., Dymerski, T., & Szweda, P. (2018). Investigation of the antifungal activity and mode of action of Thymus vulgaris, citrus limonum, pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus essential oils. Molecules, 23(5), 1116. https://doi.org/10.3390/molecules23051116
  17. Tian, F., Lee, S. Y., & Chun, H. S. (2019). Comparison of the antifungal and antiaflatoxigenic potential of liquid and vapor phase of Thymus vulgaris essential oil against aspergillus flavus. Journal of Food Protection, 82(12), 2044-2048. https://doi.org/10.4315/0362-028X.JFP-19-016
  18. Nickavar. B. (2005). Analysis of the essential oils of two thymus species from Iran. Food Chemistry, 90(4), 609-611. https://doi.org/10.1016/j.foodchem.2004.04.020
  19. Pavlyuk I., Stadnytska N., Jasicka-Misiak I., Gorka B., Wieczorek P.P., Novikov V. (2015). A study of the Chemical Composition and Biological Activity of Extracts from Wild Carrot (Daucus carota L.) Seeds Waste. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 6(2), 603-611.
  20. Stadnytska N., Fito I, Novikov V., Jasicka-misiak I., Wieczorek P. (2020). Effect of extraction solvent on total phenolic content, total flavonoid content and antioxidant activity of Cetraria islandica. International Journal of Pharm Tech Research. 13(03), 198-205. https://doi.org/10.20902/IJPTR.2019.130310