У наш час зростання кількості населення, призводить до утворення великої кількості відходів біомаси внаслідок різноманітної людської, промислової та сільсь-когосподарської діяльності. Для регулювання відходів біомаси та пом'якшення величезного спектра шкоди навколишньому середовищу застосовують переважно анаеробне бродіння. Метою цієї статті є підвищення ефективності анаеробного бродіння багатокомпонентних субстратів з використанням суміші відходів активного мулу (ВАМ), стічних вод виробництва оливкової олії (СВВОО) і гною великої рогатої худоби (ГВРХ). У плануванні експерименту використано методологію поверхні відгуку для визначення індивідуального впливу й інтерактивного ефекту на вихід метану та хімічне зниження потреби в кисні. Після числової оптимізації за допомогою Design Expert® оптимальні фактичні значення тестових факторів були такими: початкове pH = 8, співвідношення загальна хімічна потреба в кисні : загальний азот = 47, 42, співвідношення ГВРХ/ВАМ-СВВОО = 0,352, загальний сухий залишок ЗСЗ = 42,94 г/л. Отримані результати вказують, що ефективності анаеробного спільного бродіння можна досягти через оптимізацію складу субстрату для забезпечення більшого мікробного синергічного ефекту.
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