1. JCCT
  2. All Volumes and Issues
  3. Volume 13, Number 4, 2019
  4. Development of Cold Fiber Head Space Solid-Phase Microextraction for Analysis of 2,5 Hexandion in Urine

Development of Cold Fiber Head Space Solid-Phase Microextraction for Analysis of 2,5 Hexandion in Urine

JCCT.
2019;
Volume 13, Number 4
: pp. 482 - 488
https://doi.org/10.23939/chcht13.04.482
Authors:
  1. Yasaman Pourbakhshi
  2. Abdul Rahman Bahramy
  3. Farshid Ghorbani Shanha
  4. Mohammad Javad Assari
  5. Leila Tajik
  6. Maryam Farhadian
1
Center of Excellence for Occupational Health, Research Center of Health Sciences, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
2
Center of Excellence for Occupational Health, Research Center of Health Sciences, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
3
Center of Excellence for Occupational Health, Research Center of Health Sciences, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
4
Center of Excellence for Occupational Health, Research Center of Health Sciences, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
5
Center of Excellence for Occupational Health, Research Center of Health Sciences, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
6
Department of Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamedan, Iran

2,5-Hexanedione (2,5-HD), the main metabolite of n-hexane was extracted from urine samples using cold fiber head space solid-phase microextraction (CF-HS-SPME) based on thermoelectric cooling and analyzed with gas chromatography equipped with a flame ionization detector (GC-FID). Important parameters for improved efficiency of fiber sorption including sample volume, extraction time and temperature were optimized using a central composite design. The optimum conditions were determined, under which the detection limit and the relative standard deviations were found. The method was shown to be rapid, sensitive and easier than conventional methods for quantitative analysis of 2,5-HD in urine samples.

urine samples
2, 5-hexanedione
cold fiber
solid-phase microextraction
n-hexane
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