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  2. All Volumes and Issues
  3. Volume 14, Number 1, 2020
  4. Biosynthesis Products of Pseudomonas sp. PS-17 Strain Metabolites. 1. Obtaining and Thermal Characteristics

Biosynthesis Products of Pseudomonas sp. PS-17 Strain Metabolites. 1. Obtaining and Thermal Characteristics

JCCT.
2020;
Volume 14, Number 1
: pp. 26 - 31
https://doi.org/10.23939/chcht14.01.026
Authors:
  1. Ihor Semeniuk
  2. Viktoria Kochubei
  3. Volodymyr Skorokhoda
  4. Tetyana Pokynbroda
  5. Halyna Midyana
  6. Оlena Karpenko
  7. Viktor Melnyk
1
Department of PhChFF InPOCC NAS of Ukraine
2
Lviv Polytechnic National University
3
Lviv Polytechnic National University
4
Department of PhChFF InPOCC NAS of Ukraine, L.M. Lytvynenko Institute of Physical Organic Chemistry and Coal Chemistry of the NAS of Ukraine
5
Department of PhChFF InPOCC NAS of Ukraine, L.M. Lytvynenko Institute of Physical Organic Chemistry and Coal Chemistry of the NAS of Ukraine
6
Institute of Physical-Organic Chemistry and Coal Chemistry named after L. M. Lytvynenko of the National Academy of Sciences of Ukraine, Department of Technology of Biologically Active Substances, Pharmacy & Biotechnology, Lviv National Polytechnic University
7
Lviv Polytechnic National University, Department of Information Technology Security

Biogenic surfactants (rhamnolipid biocomplex, dirhamnolipid and exopolysaccharide) and polyhydroxyalkanoate biopolymer which are the new biosynthesis products of Pseudomonas sp. PS-17 strain have been obtained. The thermal transformation of these products has been studied in the air in the temperature range of 293–1273 K. Thermolysis and IR spectroscopy revealed a formation of rhamnolipid biocomplex between rhamnolipids and exopolysaccharide upon acidification of the culture fluid supernatant to pH = 3. Cellular polymer – polyhydroxyalkanoate – was identified by UV-Vis spectroscopy. According to the thermal analysis, the melting point and initial degradation temperature of polyhydroxyalkanoate were determined. Using the Vicat method, the heat resistance of the polymer was determined.

Pseudomonas sp. PS-17
biosurfactant
ramnolipids
polysaccharides
polyhydroxyalkanoate
biopolymer
thermal analysis
UV-Vis spectroscopy
IR spectroscopy
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