1. JCCT
  2. Всі випуски
  3. Випуск 16, Номер 1, 2022
  4. Виділення антибактеріального нано-гідроксиапатитного біоматеріалу з кісток буйволів та його характеристика

Виділення антибактеріального нано-гідроксиапатитного біоматеріалу з кісток буйволів та його характеристика

JCCT.
2022;
Випуск 16, Номер 1
: cc. 133–141
https://doi.org/10.23939/chcht16.01.133
Автори:
  1. Kshama Parajuli
  2. Komal Prasad Malla
  3. Nicodemus Panchen
  4. G.C. Ganga
  5. Rameshwar Adhikari
1
Central Department of Chemistry, Tribhuvan University
2
Central Department of Chemistry, Tribhuvan University, Research Centre for Applied Science and Technology (RECAST), Tribhuvan University
3
Central Department of Chemistry, Tribhuvan University
4
Department of Microbiology
5
Central Department of Chemistry, Tribhuvan University, Research Centre for Applied Science and Technology (RECAST), Tribhuvan University

Методом термічного розкладу з біовідходів (кісток буйвола) виділені наночастинкигідроксиапатиту (HAp). Отриманий білий порошкоподібний матеріал охарактеризований за допомогою інфрачервоної спектроскопії з перетворенням Фур’є (FTIR), дифракції рентгенівських променів (XRD), скануючої електронної мікроскопії (SEM) та енергодисперсійного рентгенівського аналізу (EDX). За допомогою FTIR підтверджено, що термічне оброблення кісткового порошку при температурі 1223 К або вище видаляє органічні частини, що призводить до утворення чистого неорганічного біомінералу. Рентгенофазовий аналіз показав, що отриманий матеріал єнанокристалічнимHAp (nano-HAp) із середнім діаметром зерен 25 нм, а їх паличкоподібні частинки з щільно агломерованою морфологією підтверджені аналізом SEM. Крім кальцію (Ca), фосфору (P) і кисню (O), слідові кількості алюмінію (Al), магнію (Mg), міді (Cu), цирконію (Zr) і вуглецю (C) виявлені за допомогою EDX. Антибактеріальну активність nano-HAp проти шести стандартних штамів досліджували методом дифузії. В інтервалі досліджуваних концентрацій встановлено, що найбільшу активність nano-HAp виявляє доAcinetobacterbaumannii, меншу активність доEscherichiacoli, Pseudomonasaeruginosa та Staphylococcusaureus, і зовсім неактивний щодо Salmonellatyphi та Staphylococcusaureus, стійкого до метициліну (MRSA).Показано, щонано-HAp потенційно можна застосовувати в біомедицині.

кістки буйвола
гідроксіапатит
наноматеріал
антибактеріальна активність
біокераміка
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